Frequently Asked Questions (FAQ)
ISB-CGC Accounts and Cloud Projects
Do I have to request an ISB-CGC account before I can try the web app?
No, you can just “sign in” to the Web App using your Google identity.
I want to be able to run big jobs using Google Compute Engine on the TCGA data hosted by the ISB-CGC. What should I do?
You will need to request a Google Cloud Platform (GCP) project. Please see How to Request Cloud Credits for more details about requesting a project.
Can I use any email address as a Google identity?
Yes, you can. If your email address is not already linked to a Google account, you can create a Google account with your current email address. Please note, however that although these two accounts will then share the same name, they will still be two separate accounts, with two separate passwords, etc. (It is also possible that your institutional email address is already a Google account, if your institution uses Google Apps. This is how to find out).
How do I connect my Google Cloud Project to the ISB-CGC?
Your Google Cloud Project gives you access to all of the technologies that make up the Google Cloud Platform. These technologies include BigQuery, Cloud Storage, Compute Engine, etc. The ISB-CGC makes use of a variety of these technologies to provide access to the TCGA data, as well as many other data sets. Please see the Google Cloud Project Setup and Data Access section in the Quick Start Guide.
The connection between your Google Cloud Project (whether it is an ISB-CGC sponsored and funded project or your own personal project) and the ISB-CGC is your Google identity (also referred to as your “user credentials”).
Access to all ISB-CGC hosted data is controlled using the Data Commons Framework Gen3 which defines the permissions attached to each data set, bucket, or object.
What project information do I input on the Register a Google Cloud Project page?
You will need to input the Google Cloud Project ID which can be found on the Dashboard page of the Google Console under Project info.
Why do I add the service account email@example.com to my Google Cloud Project?
This service account is needed in your Google Cloud Project IAM page for the ISB-CGC project to be able to automatically verify that all users of your Google Cloud Project have the same appropriate access rights to the protected data that has been requested for the project.
What service account do I use on the Register a Service Account page to be able to gain access to protected data?
On the Register a Service account page you are asked to input a service account ID. You need to go to the IAM and Admin page which can be found in your console for your Google Cloud Project to find the correct service account. The service account you would like to use is named, “Compute Engine default service account”. This service account is the default option on the Register A Service Account page. Please DO NOT use the service account firstname.lastname@example.org (you will be prevented from using this account by our software and an error message will be sent indicating this).
What happens if I accidently delete the default service account from a Google Cloud Project?
If you accidently delete the default service account associated to the Google Cloud Project you are working in, you can no longer authorize the service account during instance creation, associate the service account to controlled access data, and many other functionalities will no longer work.
If you then try to add the service account back to the Google Cloud Project, this error occurs:
ERROR: (gcloud.compute.instances.create) Some requests did not succeed: - The resource ‘xx…@project.gserviceaccount.com’ of type ‘serviceAccount’ was not found.
Unfortunately at this time, there is no direct way to recover the default service account.
One workaround to recreate the Google Compute Engine default service account is to disable and reenable Google Compute Engine API in your project. This will only work if you have no Google Compute Engine resources (e.g VMs, Disks, Snapshots etc) in your project; otherwise, you will get “Backend Provisioning Error” when you try to disable Compute Engine API.
Another solution would be creating a new project and redeploying your instances there.
Google has an internal feature request to prevent accidental deletion of default service accounts.
There is a Google forum discussion that can be found here with more details and explanation.
ISB-CGC Web Interface
I ran the same query in the Web App that I’ve run before, but the results were different. Why is that?
The Web App performs its data retrieval and counts on ISB-CGC Google BigQuery tables which are based on the latest GDC data release. So, it’s possible that a new GDC release occurred since you last performed that query.
Why do I sometimes get a “Do you want to leave this site?” pop-up box when leaving a page or canceling a workflow edit?
This is a security feature when working with forms found in most web browsers; it lets you know that you may have made some changes which will be lost when you navigate away from the page. If you intend to cancel what you were doing, you can safely ignore it.
Which web browser is recommended when working with the site?
We recommend using Google Chrome browser. Currently a chart will display slightly off when working with workbooks on a FireFox browser.
Why did I get a 401 error on the IGV Browser?
You will see the 401 error only if your pop-up blocker is enabled for the ISB-CGC website. Please disable the pop-up blocker on the top right-hand side of the screen by selecting to always allow pop-ups from ISB-CGC.
Why does the web browser crash if too many IGV Browser tabs are opened at once?
The web browser may crash when too many IGV Browser tabs are open due to the memory intensive nature of loading bam files. When working with the IGV Browser, please be mindful of having multiple tabs of the IGV Browser open.
Does SeqPeek and CNVR plotting only work with TCGA data?
We currently have no data associated with CNVR or Seqpeek for TARGET or CCLE. Therefore, SeqPeek and CNVR will only work with TCGA data.
Where can I find the TCGA data that ISB-CGC has made publicly available in BigQuery tables?
The BigQuery web interface can be accessed at https://console.cloud.google.com/bigquery. If you have not already added the ISB-CGC data sets to your BigQuery “view”, click on the blue arrow next to your project name at the top of the left side-bar, select “Switch to Project”, then “Display Project…”, and enter “isb-cgc-bq” (without quotes) in the text box labeled “Project ID”. For older ISB-CGC data sets, repeat and enter “isb-cgc”. All ISB-CGC public BigQuery data sets and tables will now be visible in the left side-bar of the BigQuery web interface. Note that in order to use BigQuery, you need to be a member of a Google Cloud Project.
How can I apply for access to low-level DNA and RNA sequence data?
In order to access the TCGA or All other controlled-access data available, you will need to apply to dbGaP. Please also review our section on Understanding Data Security.
Can I authenticate to NIH programmatically?
No, the current NIH authentication flow requires web-based authentication and must therefore be done from within the ISB-CGC Web App. Once you have authenticated to NIH via the Web App, and your dbGaP authorization has been verified, the Google identity associated with your account will have access to the controlled-data for 24 hours.
I get a different number of samples in BigQuery than I do with the same query in the Web App. Why?
Older programs like TCGA have both legacy data (data from the original program) and harmonized data (data run through the Genomics Data Commons). The Web App primarily uses harmonized data whereas BigQuery contains both legacy and harmonized data. In addition, some cases and samples have been removed from the Web App if annotation suggests the data from those cases or samples are incorrect, misleading or from cases of uncertain origin. Most of these cases and samples are still in BigQuery and users are encouraged to check the annotations tables.
I want to write Python scripts that access the TCGA data hosted by the ISB-CGC. Do you have some examples that can get me started?
Yes, of course! The best place to start is with our Community Notebooks or our repository in GitHub. You can run any of these examples yourself. It includes an introduction explaining what Notebooks are, how to get started as a novice user, and how to run more advanced analyses once you are comfortable.
I want to use R and Bioconductor packages to work with the TCGA data. How can I do that?
You can run RStudio locally or deploy a dockerized version on a Google Compute Engine VM. You can find some great examples to get you started in our Community Notebooks or our repository in Community Notebooks GitHub.
For an example on how to use Bioconductor packages with TCGA data in BigQuery, please check out our interactive tutorial found here.
Regulome Explorer Users
Can I run Regulome Explorer Analyses using TCGA tables of heterogeneous data in BigQuery?
Yes, of course! A series of Python Notebooks have been created to replicate Regulome Explorer and includes detailed information on the statistical methods implemented. To get started, please visit our Regulome Explorer page in readthedocs or our Repository in Regulome Explorer GitHub.